Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (E. coli) |
JK10 | this paper | none | genotype: ∆cyaA ∆cpdA
∆lacY ∆lacZ ∆dksA |
| Recombinant DNA reagent |
pJK47.419 |
this paper |
none |
cloning vector with BsmBI cut sites, ccdB cassette, lacZ reporter gene, kanamycin resistance, pSC101 origin |
| Recombinant DNA reagent |
pJK48 and variants |
this paper | none | reporter plasmids cloned from pJK47.419 |
| Chemical compound |
cAMP | Sigma-Aldrich | A9501-1G | Adenosine 3’,5’-cyclic monophosphate, 1 gram |
| Chemical compound |
IPTG | Sigma-Aldrich | I5502-1G | Isopropyl β-D-1- thiogalactopyranoside, 1 gram |
| Chemical compound |
ONPG | Sigma-Aldrich | N1127-5G | 2-Nitrophenyl β-D-galactopyranoside, 5 gram |
| Commercial assay or kit |
PureLink Genomic DNA Mini Kit |
ThermoFisher | K182001 | none |
| Commercial assay or kit |
Nextera XT DNA Library Preparation Kit |
Illumina |
FC-131–1024 |
24 samples |
| Other | RDM | Teknova | M2105 | growth media: MOPS EZ Rich Defined Medium Kit, 5 liter |
| Other | PopCulture Reagent |
MilliporeSigma | 71092–4 | 75 milliliters |
| Other | Breathe-Easier film | USA Scientific | 9123–6100 | sterile, 100 per box |
| Other | Epoch 2 Microplate Spectrophotometer |
BioTek |
EPOCH2C |
none |
| Software | analysis scripts | this paper | none | Available at https://github.com/jbkinney/17_inducibility
(copy archived at https://github.com/elifesciences-publications/17_inducibility) |