Key resources table.
| Reagent type | Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Saccharomyces cerevisiae) |
MBF1/
YOR298C-A |
SGD:S000007253 | ||
| Gene (S. cerevisiae) |
ASC1/
YMR116C |
SGD:S000004722 | ||
| Gene (S. cerevisiae) |
RPS3/
YNL178W |
SGD:S000005122 | ||
| Strain, strain background (S. cerevisiae, MATa) |
BY4741 | Open Biosystems |
||
| Strain, strain background (S. cerevisiae, MATα) |
BY4742 | Open Biosystems |
||
| Genetic reagent (S. cerevisiae) |
See Supplementary table 1 in Supplementary file 3 |
this paper | ||
| Antibody | anti-HA High Affinity (Rat monoclonal) |
Roche | 3F10, catalog# 11867423001; RRID:AB_10094468 |
1:3000 dilution |
| Antibody | Goat anti-Rat IgG-HRP conjugate |
Jackson Immuno Research |
catalog# 112-035-003; RRID:AB_2338128 |
1:5000 dilution |
| Antibody | anti-G-6-PDH (Rabbit monoclonal) |
Sigma | catalog# A9521; RRID: AB_258454 |
1:5000 dilution |
| Antibody | Goat anti- Rabbit IgG- HRP conjugate |
Biorad | catalog# 1706515; RRID:AB_11125142 |
1:10000 dilution |
| Recombinant DNA reagent |
See Supplementary table 2 in Supplementary file 3 |
this paper | ||
| Sequence- based reagent |
See Supplementary table 3 in Supplementary file 3 |
this paper | ||
| Sequence -based reagent |
Random Primers |
Invitrogen | catalog# 48190011 |
|
| Commercial assay or kit |
T4 DNA Polymerase, LIC-qualified |
Novagen | catalog# 70099 |
Used for ligation- independent cloning |
| Commercial assay or kit |
Super Script II Reverse Transcriptase |
Invitrogen | catalog# 18064014 |
|
| Commercial assay or kit |
RQ1 Rnase- Free Dnase |
Promega | catalog# M6101 |
|
| Commercial assay or kit |
RiboMAX Large Scale RNA Production System-T7 |
Promega | catalog# P1300 | |
| Commercial assay or kit |
MicroSpin G-25 columns |
GE | catalog# 27-5325-01 |
|
| Commercial assay or kit |
Fast SYBR Green Master Mix |
Applied Biosystems |
catalog# 4385612 |
|
| Commercial assay or kit |
Glutathione sepharose-4B |
GE | catalog# 17-0756-01 |
|
| Commercial assay or kit |
MagStrep ‘type3’ XT beads |
IBA | catalog# 2-4090-002 |
|
| Chemical compound, drug |
5-FOA | USBiological | catalog# F5050 |
|
| Chemical compound, drug |
Complete mini EDTA-free protease inhibitor |
Roche | catalog# 11836170001 |
|
| Chemical compound, drug |
leupeptin | Roche | catalog# 11017128001 |
|
| Chemical compound, drug |
pepstatin | Roche | catalog# 11359053001 |
|
| Chemical compound, drug |
Glutathione | Sigma | catalog# G4251 |
|
| Chemical compound, drug |
Biotin | IBA | catalog# 2-1016-002 |
|
| Software, algorithm |
MultAlin | (Corpet, 1988) | http://multalin.toulouse.inra.fr/multalin/ |