Figure 1.

CRMP4a overexpression reduces cytoskeleton organization in prostate cancer cells. A. PC-3 cells stably infected with lentiviruses harboring the control empty vector or CRMP4a expression constructs were harvested for western blot with the antibodies as indicated. Actin blot served as protein loading control. B. PC-3 stable subline cells as indicated (empty vector or CRMP4a) were seeded on cover glass in full culture media for 24 h and then stained with iFluor555-conjugated phalloidin (red) and Hoechst33342 (blue). Cells were also subjected to immunocytofluorescent staining with anti-vinculin antibodies (green) followed by visualization with AlexaFluor^®488-labeld secondary antibodies. The representative microscopic images were shown from four independent experiments. C. Quantitative data for focal adhesion area or number per cell, percentage of cell area, and lamellipodia numbers per cell formation were shown as mean ± SEM. The asterisk indicates a statistical significance compared to the control (student’s t-test, p < 0.01).