Figure 6.

RhoA activation blocks CRMP4a-mediated suppression of cell spreading. A. PC-3 cells stably infected with empty vector, CRMP4a or CRMP4a plus RhoA-Q63L lentiviruses were plated in glass-bottomed culture dish for living cell system. Time-lapse sequential images were captured at intervals of 5 min for 3 h using the IX2-ZDC2 laser-based autofocusing system. B. Cell migration trajectories were determined using the ImageJ software. The movie files were opened in ImageJ program and cell migrate trajectories were tracked along the centrality of cells with the “add” button in plugin ‘MTrackJ’. The lengths of cell migrate trajectories were calculated using the “measure” button in plugin ‘MTrackJ’. C. Quantitative data for trajectory length and perimeter were shown as mean ± SEM from three independent microscopic fields. The asterisk indicates a significant difference compared to the vector control (student’s t-test, p < 0.05).