MEG3 up-regulation suppressed cell apoptosis related protein expression in 5637 cells and T-24 cells. (A-B) After being transfected with pcDNA3.1-MEG3, mRNA and protein expression level of apoptosis related protein Bcl-2 and Cyclin D1 was decreased, while the mRNA or protein expression of Bax, cleaved-caspase 3 were both increased after being examined by RT-qPCR and western blot. *P < 0.05, **P < 0.01, compared with pcDNA3.1-NC. Each test was repeated three times.