Figure 5.

MiR-96 was a target of MEG3 in 5637 cells and T-24 cells. (A) RT-qPCR showed that MEG3 and miR-96 expression in 45 paired tissues and revealed that MEG3 and miR-96 were negatively related; (B) MiRcode predicted that MEG3 and miR-96 might have a binding site; (C) Dual luciferase reporter assay proved that MEG3 bound to miR-96 directly. The group which binding site on MEG3 was mutated (mut group) had no significant difference after being treated with miR-96 mimics; (D) RT-qPCR demonstrated that pcDNA3.1-MEG3 could suppress the expression of miR-96; (E) RT-qPCR showed that miR-96 mimics could not affect the production of MEG3. *P < 0.05, **P < 0.01, compared with NC. Each test was repeated three times.