The dual-luciferase reporter gene assay shows that miR-129-5p targets ZIC2.
Note: A, predicted binding sites of miR-129-5p to the ZIC2-3ʹUTR; B, luciferase activity determined by luciferase reporter gene assay; NC, negative control; miR-129-5p, microRNA-129-5p; ZIC2, zinc finger protein of the cerebellum 2; UTR, untranslated region; *, p < 0.05 compared with the NC group.