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. 2018 Sep 27;19(12):1162–1173. doi: 10.1080/15384047.2018.1491497

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Figure 5. — RT-qPCR and western blotting illustrate the inhibition of the mRNA and protein levels of ZIC2, Shh, Gli1, and Gli2 by miR-129-5p.

Note: A, miR-129-5p level and mRNA levels of Shh, Gli1 and Gli2 in C-33A cell line; B, protein levels of ZIC2, Shh, Gli1 and Gli2 in C-33A cell line; C, protein bands of ZIC2, Shh, Gli1 and Gli2 in C-33A cell line; D, miR-129-5p level and mRNA levels of Shh, Gli1 and Gli2 in Hela cell line; E, protein levels of ZIC2, Shh, Gli1 and Gli2 in Hela cell line; E, protein bands of ZIC2, Shh, Gli1 and Gli2 in Hela cell line; NC, negative control; miR-129-5p, microRNA-129-5p; ZIC2, zinc finger protein of the cerebellum 2; Shh, sonic Hedgehog; Gli, glioma-associated oncogene family zinc finger; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; *, p < 0.05 compared with the blank and NC groups; #, p < 0.05 compared with the miR-129-5p inhibitor group.