. 2018 Sep 27;19(12):1162–1173. doi: 10.1080/15384047.2018.1491497

Table 1.

Primer sequences for RT-qPCR.

Gene	Primer sequence (5ʹ-3ʹ)
miR-129-5p	F: GATCCGCAAGCCCAGACCGCAAAAAGTTTTTA
miR-129-5p	R: AGCTTAAAAACTTTTTGCGGTCTGGGCTTGCG
ZIC2	F: GCGCAACTCCACAACCAGTA
ZIC2	R: TGCCGCATATAGCGGAAAAAG
Shh	F: CTCGCTGCTGGTATGCTCG
Shh	R: ATCGCTCGGAGTTTCTGGAGA
Gli1	F: AGCGTGAGCCTGAATCTGTG
Gli1	R: CAGCATGTACTGGGCTTTGAA
Gli2	F: CTGCCTCCGAGAAGCAAGAAG
Gli2	R: GCATGGAATGGTGGCAAGAG
U6	F: GCTTCGGCAGCACATATACTAAAAT
U6	R: CGCTTCACGAATTTGCGTGTCAT
β-actin	F: CTACAATGAGCTGCGTGTGG
β-actin	R: TAGCTCTTCTCCAGGGAGGA

Note: miR-129-5p, microRNA-129-5p; ZIC2, zinc finger protein of the cerebellum 2; Shh, sonic Hedgehog; Gli, glioma-associated oncogene family zinc finger; RT-qPCR, reverse transcription quantitative polymerase chain reaction.