Figure 3.

Sorafenib inhibits SUMO1 mediated SUMOylation of p65(A) Western blot analysis indicates that sorafenib decreases SUMO1. HepG2 and HCCLM3 cells were incubated with 10 µm sorafenib for 24h. Protein levels of SUMO1 were analyzed by western blot and normalized to GAPDH. (B) Co-immunoprecipitation experiments indicate sorafenib inhibits the interaction of p65 and SUMO1. (C) Immunofluorescences staining p65 and SUMO1 were performed. Data are means ± SEM from 3 independent experiments, * means p < 0.05, ** means p < 0.01, *** means p < 0.001 by unpaired student T test.