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. 2018 Sep 6;19(11):973–983. doi: 10.1080/15384047.2018.1450112

Table 2.

The primers for conduction of qRT-PCR.

Name Forward Primers Reverse Primers
GAPDH 5′-AACGGATTTGGTCGTATTG-3′ 5′-GGAAGATGGTGATGGGATT-3′
HOXA10 qPCR 5'-GGGTAAGCGGAATAAACT-3' 5'-GCACAGCAGCAATACAATA-3'
U6 5'-CTCGCTTCGGCAGCACA-3' 5'-AACGCTTCACGAATTTGCGT-3'
miR-135a-5p 5′-AACCCTGCTCGCAGTATTTGAG-3′ 5′-GCGGCAGTATGGCTTTTTATTCC-3′
HOXA10 3′UTR 5′-GAATTCTGGTCCCCTCCCTCTGTCTT-3′ 5′-CTCGAGATCGTCTTTTTCCACGCACAG-3′
HOXA10 MUT 5′-GGCTGGTTTGTTCTGAAGAGATAGATATTGT-3′ 5′-ACAATATCTATCTCTTCAGAACAAACCAGCC-3′
HOXA10 CDS 5′-GAATTCATGTCAGCCAGAAAGGGCT-3′ 5′-CTCGAGCCGCGTCGCCTGGAGATT-3′