Name |
Forward Primers |
Reverse Primers |
GAPDH |
5′-AACGGATTTGGTCGTATTG-3′ |
5′-GGAAGATGGTGATGGGATT-3′ |
HOXA10 qPCR |
5'-GGGTAAGCGGAATAAACT-3' |
5'-GCACAGCAGCAATACAATA-3' |
U6 |
5'-CTCGCTTCGGCAGCACA-3' |
5'-AACGCTTCACGAATTTGCGT-3' |
miR-135a-5p |
5′-AACCCTGCTCGCAGTATTTGAG-3′ |
5′-GCGGCAGTATGGCTTTTTATTCC-3′ |
HOXA10 3′UTR |
5′-GAATTCTGGTCCCCTCCCTCTGTCTT-3′ |
5′-CTCGAGATCGTCTTTTTCCACGCACAG-3′ |
HOXA10 MUT |
5′-GGCTGGTTTGTTCTGAAGAGATAGATATTGT-3′ |
5′-ACAATATCTATCTCTTCAGAACAAACCAGCC-3′ |
HOXA10 CDS |
5′-GAATTCATGTCAGCCAGAAAGGGCT-3′ |
5′-CTCGAGCCGCGTCGCCTGGAGATT-3′ |