Figure 5.

High throughput screen of targeted agents against HGG neurospheres. (A) Heat map shows sensitivity to each chemotherapeutic drug as a measure of IC50 with all drugs being tested up to 10 µM for 72h. Each value is depicted as a gradient of red to white with white being the highest dose tested and red a low nM-range concentration while varying shades of pink indicate an intermediate IC50 value. Each number corresponds to a different chemotherapeutic agent, identified in the adjacent table. (B) Dose response curves for temozolomide, temsirolimus, BI2536 and ceritinib. Each drug was tested over a range of concentrations 0.001–10 µM for 72h and viability was compared to DMSO treated cells.