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. 2018 Sep 27;19(12):1128–1138. doi: 10.1080/15384047.2018.1480293

Figure 5.

Figure 5.

Cisplatin induced the phosphorylation of Chk1 and Chk1 phosphorylation is RNF138 dependent. (A, B) Immunoblotting analysis of the RNF138 and phosphorylation of Chk1 signaling pathway level in AGS and SGC7901 cells treated with cisplatin. α-Tubulin was used as the loading control. (C, D) Immunoblotting analysis of the RNF138 and Chk1 signaling pathway level in isogenic AGS and AGS/DDP (C) and SGC7901 and SGC7901/DDP (D) cell lines treated with cisplatin. GAPDH was used as the loading control. (E) Chk1 signaling pathway was detected in AGS and SGC7901 stable cell lines treated with cisplatin by immunoblotting detection. α-Tubulin was used as the loading control. (F) Chk1 signaling pathway was detected in AGS/DDP and SGC7901/DDP cells transfected with siRNA treated with cisplatin by immunoblotting detection. α-Tubulin was used as the loading control. Data were normalized to control-treated cells. The numbers below the panels represent the normalized protein expression levels.