Figure 6.

The stability of RNF138 is increased in cisplatin-resistant GC cells. (A, B) Half-life of RNF138 was detected in isogenic AGS, AGS/DDP and SGC7901, SGC7901/DDP GC cell lines by CHX assay. RNF138 and α-Tubulin signals in the immunoblotting detection were quantified by densitometry. The ratios of RNF138/α-Tubulin were normalized to time zero and plotted against time. (C) Schematic model for the mechanism by which RNF138 contributes to cisplatin resistance by facilitating activation of the Chk1 pathway in GC cells.