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. 2018 Dec 20;8:17988. doi: 10.1038/s41598-018-36211-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A–D) GUS assay of leaf and stem section of transgenic and non-transgenic M. cordata plants. (A,B) Staining of GUS activity in overexpression McBBE-OX plants. (C) Staining of GUS activity in transgenic PsBBE-OX plant. (D) Staining of GUS activity in non-transgenic plant. (E–G) Amplify of GUS, nptII, PsBBE genes in transgenic and non-transgenic plants, marker 2 kb plus ladder and vector1 (pCMBIA-2301), vector2 (pCMBIA-2301 + PsBBE), vector3 (pCMBIA-2301 + McBBE) were used to positive control, WT negative control. (E) Amplify GUS gene (750 bp). (F) Amplify nptII gene (364 bp). (G) Amplify PsBBE gene (750 bp). Vec1-Vec3 means vector1 to vector3. WT means wild type.