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. 2018 Dec 20;8:17988. doi: 10.1038/s41598-018-36211-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The analysis of alkaloid levels (from (S)-norcoclaurine to SAN and CHE) in transgenic (pcambia2301 + McBBE, pcambia2301 + PsBBE, pcmbia2301) and WT M. cordata plants were negative control. Data represent means ± SD (n = 3). Asterisks denote the significant changes (* means P < 0.05, ** means P < 0.01, *** means P < 0.005, **** means P < 0.001). McMSH and McSPS genes in this pathway still uncharacterized, we mark it in blue. EV means empty vector, WT means wild type.