Table 1.
Estimation of L-asparaginase activity in SLP, stability, kinetics and pH studies.
| Parameter/Condition tested | Enzyme preparation tested | |
|---|---|---|
| Stability when stored at 25 °C | L-asp | LPE |
| Enzyme activity Day 1 (IU/mL) | 24.55 ± 1.12 | 30.15 ± 1.56 |
| Enzyme activity Day 25 (IU/mL) | 2.62 ± 0.55 | 29.47 ± 2.74 |
| Kinetics for natural substrate L-asparagine | L-asp | LPE |
| Vmax (µmole/mL/min) | 7.79 ± 0.34 | 10.21 ± 1.43 |
| Km (mM) | 0.070 ± 0.01 | 0.04 ± 0.001 |
| Optimum pH for activity | L-asp | LPE |
| Enzyme activity at pH 8.5 | 6.12 ± 0.21 | 7.70 ± 0.38 |
| Enzyme activity at pH 9 | 5.50 ± 0.24 | 8.15 ± 0.36 |
| Trypsin tolerance test (half life) | L-asp | LPE |
| Time (mins) | 50.19 | 120.56 |