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. 2018 Dec 20;8:18017. doi: 10.1038/s41598-018-35755-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Antibody response from Adenovirus vaccination. (A–D) C57BL/6 mice (n = 5) were immunized with either Ad5-prM-E, Ad4-prM-E, or sham vaccine according to the timeline in (A) and at the indicated dose via the i.m. route (B). ZIKV E protein specific antibodies were measured using an ELISA in (C) and neutralization titer (PRNT50) was determined by plaque reduction assay in (D) (^*p < 0.05; ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001; one-way ANOVA with Bonferroni multiple comparisons). To ensure that the undetectable antibody response to Ad4-prM-E vaccination was not sue to differences in infectivity of Ad vaccine virus stocks, the virus particle (vp) to infectious unit (IFU) ratio was determined (E). C57BL/6 mice (n = 5) were then vaccinated with an equal IFU dose and the antibody response measured via ELISA (F) and plaque reduction assay (G) ^**p < 0.01; one-way ANOVA with Bonferroni multiple comparisons). Data are expressed as the mean with standard error (SEM).