Table 6.

Sequences of the primers used in qRT-PCR.

Genes	Forward primers (5′–3′)	Reverse primers (5′–3′)	Annealing	Tm	Amplicon
			temp. (° C)	(° C)	size (bp)
TNF-α	ACGCTCTTCTGTCTACTG	GGATGAACACGCCAGTCG	54	52.68	212
IL-10	GAAGTGATGCCCCAGGCAGA	ACGTAGGCTTCTATGCAGT	54	58.88	253
IL-6	CATGTTCTCTGGGAAATC GTGG	AACGCACTAGGTTTGCCGA GTA	54	54.84	209
IL-1β	CCAGGATGAGGACCCAAG	TCCCGACCATTGCTGTTT	54	52.60	249
iNOS	CCCAGCCTCAAGTCTTATTTCCTC	GCACTCAGCAGCAAGTTCCATC	54	57.38	220
COX-2	CCTGTGCCTGATGATTGC	CTGATGCGTGAAGTGCTG	54	50.32	96
GAPDH	GCAAGTTCAACGGCACAGCACAG	GCCAGTAGACTCCACGACAT	54	58.84	124