FIGURE 6.

Suppression of vancomycin sensitivity in Δspr mutant backgrounds. (A) Genomic visualization of vancomycin tolerant suppressor mutations with the bars representing genomic deletions, except for tsp T479R where the bar points to the position of a point mutation. (B) Wild-type and the Δtsp suppressed mutants harboring either vector control pBAD30 or full complementation pBAD30::tsp were serially diluted and spread on TY agar plates, or TY agar plates supplemented with 20 μg/ml or 40 μg/ml of vancomycin, either containing L-arabinose or not. Images are representative of three replicates each.