Table 2.
Oligonucleotides for construction of strains and diagnostic PCR.
| FSprrec | CGATATTTATCGTTAAGGACTTCAAGGGAAAACAAACAAC GTGTAGGCTGGAGCTGCTTC |
| Rsprrec | TCTCATCAGGTAAGCCAAGGGAGGTGCTGCCTGATGAAGA CATATGAATATCCTCCTTAG |
| Fspr(c) | GAA TTG TCT CAA GCT GTG CAG G |
| Rspr(c) | ATT CGG CAA AAC GGG TTC AG |
| FYebArec | TGCGAGCTGCCTGAAAGGAGATTAACGAGGAAGTGAATAC GTGTAGGCTGGAGCTGCTTC |
| RYebArec | AGC CGG CAC ACA TCG CGT ACC GGC TCT GTC AGC GCA TTT G CATATGAATATCCTCCTTAG |
| FYebA(c) | TTA GCC AAC CAG TAT GCG AGC |
| RYebA(c) | GTA GCG ACG TCT GCG TCT C |
| FYdhOrec | GTAGATTAGAATTATCAGGTTTTGTAAATCATACGCAGGC GTGTAGGCTGGAGCTGCTTC |
| RYdhOrec | AAG AAG AAG TTA TCC TGT CGT TAA ACG ACA GGA TAA AAT A CATATGAATATCCTCCTTAG |
| FYdhO(c)2 | CTG AAG CCT GTC ATT GTA ACG G |
| RYdhO(c)2 | CGA TCT CTT CCA GCG ATT TGC |
| FTsprec | ATGTCTTTGATTGTACGCGCAGAACACCTGGTGTTCTGAA GTGTAGGCTGGAGCTGCTTC |
| RTsprec | TTA AAA AAA AAC AGG CAC AAT TTT TTG TGC CTG TTT AGC GCATATGAATATCCTCCTTAG |
| FTsp(c) | TCA CCA AAG ATG GTG TCC GT |
| RTsp(c) | TAT CCT GAC GAC TTC TGC GC |
| FRfaCrec | GCAGCGGGTTCTGGAAGAGCTTCATTCGCTGTTGTCGGAA GTGTAGGCTGGAGCTGCTTC |
| RRfaCrec | TCT TTT CTC CAC AAT AGG TTT GGG ATG AGA CAG AGT CTC T CATATGAATATCCTCCTTAG |
| FRfaC(c) | AAG TGC GTA AAG GTG ATA CGG |
| RRfaC(c) | CGC TTT ATT CCA GAT CGG CTT |
| FRfaPrec | GATTTATACAGCTTACCGGAGAAGGCCGCGGATATTATTA GTGTAGGCTGGAGCTGCTTC |
| RRfaPrec | CTC ACT CAT AAA TTA CTC ACT GAG TGC ATA ATT ATT ATA A CATATGAATATCCTCCTTAG |
| FRfaP(c) | ACA CAG CCT TCC TTA CGC AA |
| RRfaP(c) | GCC AGC AGG TGT GGC AAT ATA |
| FRfaGrec | GACGGAAAAAATGCTGCCGCATGAGGCACGCACCATAGAT GTGTAGGCTGGAGCTGCTTC |
| RRfaGrec | ATC TTT ACC GCG CCA TAG TGT GGT TAA CGG CGC TTT CAG CCATATGAATATCCTCCTTAG |
| FRfaG(c) | TAC CTT TCC GTT ATT CCG GCT G |
| RRfaG(c) | GTC TCC AGC TCT CTG AAC AC |
| FMepArec | ATCGGGCACAGAATGCGGATGTAAAGACAGAGATTCCACG GTGTAGGCTGGAGCTGCTTC |
| RMepArec | AGC AGC GGG GAG ACC ATA AAC AGA TCA TAA AAA TTG TCC A CATATGAATATCCTCCTTAG |
| FMepA(c) | AGT GCC GAT CGC AGA AG |
| RMepA(c) | AAA TCC TGC CAG TAC GGC |
| FNlpCrec | CAGGTAATTTCGACGCTAAATTAATACCAAAATAAAAACA GTGTAGGCTGGAGCTGCTTC |
| RNlpCrec | ATG TTA AAA ATA GAC TAT AAA ATT TAT ATC GTC TGC GAG GCATATGAATATCCTCCTTAG |
| FNlpC(c) | CGT CGA GGG GCA TCC AAT |
| RNlpC(c) | AGT TCA ACC GGC GAT ATG TT |
| FPbpGrec | AGCTCAGGCGGTGTGCGTTACGACGCGCGTGAATCATTAT GTGTAGGCTGGAGCTGCTTC |
| RPbpGrec | TGA AGC CCG GCG GCG CGA TGC CTG CCG GGC CTG CGG CGA C CATATGAATATCCTCCTTAG |
| FPbpG(c) | TTC TGT AGC GGC AAC GCT |
| RPbpG(c) | GCG GAA ATT CTG GCA GGA A |
| FsprSacI2 | CATGAGCTCAGGAGGA CAA CAT GGT CAA ATC TCA ACC G |
| RsprHindIII | CATGAAGCTT TTA ACT GCG GCT CAG AAC TC |
| FSprC68S (Primer for making C70S) | GCAGCACTAAGAAAGGCGTCGACTCTTCC AGC TTT GTA CAG CGC ACC TTC |
| RSprC68S (Primer for making C70S) | GAA GGT GCG CTG TAC AAA GCT GGA AGA GTC GAC GCC TTT CTT AGT GCT GC |
| pBAD30Forward | TTA GCG GAT CCT ACC TGA CG |
| FTspEcoRI | CATGAATTCAGGAGGA TGT TCT GAA ACG GAG GCC A |
| RTspHindIII | CATGAAGCTT TTA CTT ATT GGC TGC CGC CT |
| FProQrec | CTGTTCATGCCTGCTGCTTGTTGGCTACGTCCGTTGTAAT GTGTAGGCTGGAGCTGCTTC |
| RProQrec | AAG CCT AAA AAA AGT GTT CAT GCC AGG CCT GGC CTC CGT TCATATGAATATCCTCCTTAG |
| FProQ(c) | GTC GCA GGA TAA TCA ACG GA |
| RProQ(c) | CGT AAT ATC TTC CAC GGC GAA G |
| FHtpXrec | CATACGATGTGGGTAATCGCATAGTGCGCTTTGTTAAATT GTGTAGGCTGGAGCTGCTTC |
| RHtpXrec | GCG TCA TTC GAC GCG CTT TTC ATA CTT GCC AGT GGG CTT ACATATGAATATCCTCCTTAG |
| FHtpX(c) | TTT CTC GTG ACT TAC CGC CT |
| RHtpX(c) | CGG TAG TGA GCG GTT TAC GTA |
| ΔnlpI-mutant | Rouf SF, 2011, J. Bac |