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. 2018 Dec 14;8:434. doi: 10.3389/fcimb.2018.00434

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Copyright © 2018 O'Neill, Skilton, Pearson, Filardo, Andersson and Clarke.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Plasmid maps of vectors constructed in this study. (A) E. coli vector pSP73::trpRBA and (B) shuttle vector pCDS5KO::trpRBA. trpA, trpB, and trpR indicate the genes of the tryptophan biosynthesis pathway, from Soton D1. Ori is the E. coli origin of replication. AmpR is the gene for ampicillin/penicillin resistance. CmR is the chloramphenicol acetyl-transferase gene for chloramphenicol resistance; RSGFP is the green fluorescence protein, and each of the chlamydial plasmid genes CDS1-8 are shown (except CDS5, which is deleted in pCDS5KO).