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. 2018 Dec 14;8:434. doi: 10.3389/fcimb.2018.00434

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Copyright © 2018 O'Neill, Skilton, Pearson, Filardo, Andersson and Clarke.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Growth curve analysis of C. trachomatis A2497 strains used in this study: wildtype (WT), plasmid cured (P-), and carrying the pSW2::GFP plasmid (GFP). (A) One-step infectivity assay; this is plotted on a log₁₀ Y axis; (B) normalised infectivity assay. Here, the inclusion counts (IFUml-¹) were normalised by dividing each value by the starting IFUml-¹ to make all curves relative to 1 IFUml-¹ for ease of comparison. (C) Confocal images of immunofluorescently stained inclusions at selected time points.