Figure 2.

mPGES-1-deficiency in T effector cells protects against colitis. (A) Weight loss in Rag1^−/− mice that received transfer of 1 × 10⁶ CD4⁺CD25⁻CD45RB^hi T cells (Teff) from WT or mPGES-1^−/− donors. The dashed lines correspond to mice that received WT Teff and WT or mPGES-1^−/− CD4⁺CD25⁺ (Treg) co-transfers. (B) Colon pathology scores from cohorts receiving transfers of WT or mPGES-1^−/− Teff cells. Flow cytometry analysis of the (C) mesenteric lymph nodes (mLN) and (D) colon lamina propria (cLP) CD4⁺ populations at the end of the experiment (week 10), with representative dot plots indicating intracellular expression of RORγt and FoxP3 and graphs below indicating summarized results from 4 experiments. ** indicates a significant difference with P < 0.05 using a two-tailed heteroscedastic Student's T-test between the WT and mPGES-1^−/− Teff donor groups.