Figure 5.

Paracrine mPGES-1-deficiency in non-lymphoid cells facilitates colitis by inhibiting generation of CD4⁺FoxP3⁺ cells. (A) Weight loss in Rag1^−/− or Rag1^−/− x mPGES-1^−/− mice that received a transfer of WT Teff donor cells. (B) Segregated colon pathology scores from both cohorts. Flow cytometry analysis of (C) mLN and (D) cLP CD4⁺ populations at the end of the experiment (10 weeks), indicating intracellular expression of RORγt and FoxP3 in summarized results from 3 experiments. (E) Fluorescence microscopy analysis of colon sections denoting CD4⁺ cell infiltrates. Blue = DAPI, Gray = CD3ε, Green = RORγt, and Red = FoxP3. **P < 0.01 and *P < 0.05 using in a one-way ANOVA with Welch's correction.