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. 2018 Dec 14;11:459. doi: 10.3389/fnmol.2018.00459

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Copyright © 2018 Tsvetkov, Roman, Baksheeva, Nazipova, Shevelyova, Vladimirov, Buyanova, Zinchenko, Zamyatnin, Devred, Golovin, Permyakov and Zernii.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Zn²⁺ binding to NCS-1 according to equilibrium dialysis experiments. The NCS-1 (5 μM) sample was pre-equilibrated with (2–150) μM Zn²⁺ by equilibrium dialysis (3.5 kDa MWCO) at 25°C. The concentrations of NCS-1-bound zinc ([Zn²⁺]_bound) and free zinc ([Zn²⁺]_free) in the resulting solutions were measured by electrothermal atomization AAS, using the absorption bands at 213.9 nm or 307.6 nm. The solid curve approximates the experimental data by Hill equation.