FIGURE 2.

Thermodynamics of calcium, zinc and magnesium binding to NCS-1. Typical ITC curves (upper panels) and binding isotherms (lower panels) representing titration of NCS-1 (25 μM) by different cations. (A) Binding of Ca²⁺. (B) Binding of Zn²⁺. (C) Binding of Mg²⁺. (D) Binding of Ca²⁺ in the presence of 250 μM Zn²⁺. (E) Binding of Zn²⁺ in the presence of 1 mM Ca²⁺. (F) Binding of Mg²⁺ in the presence of 250 μM Zn²⁺. (G) Binding of Ca²⁺ in the presence of 5 mM Mg²⁺. (H) Binding of Zn²⁺ in the presence of 5 mM Mg²⁺. (I) Binding of Mg²⁺ in the presence of 1 mM Ca²⁺. Best fits are shown as solid curves (see Table 1).