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. 2018 Dec 14;11:459. doi: 10.3389/fnmol.2018.00459

FIGURE 3.

FIGURE 3

Conformational properties of NCS-1 under saturating concentrations of calcium, magnesium and zinc. (A) Representative tryptophan fluorescence spectra of 14 μM NCS-1 under metal-free conditions (1 mM EDTA) or in the presence of Ca2+ (100 μM CaCl2), Mg2+ (1 mM MgCl2), Zn2+ (100 μM ZnCl2) or their combinations. (B) The histogram demonstrating maximum position λmax (left bar) and maximal intensity Imax (right bar) of fluorescence spectrum for the data shown in panel (A). (C) Representative CD spectra of 8 μM NCS-1 under metal-free conditions or in the presence of Ca2+ (100 μM CaCl2), Mg2+ (1 mM MgCl2), Zn2+ (100 μM ZnCl2) or their combinations. (D) The histogram representing secondary structure contents (in %) for each NCS-1 spectrum shown in panel (C).