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Thermal stability of NCS-1 (14 μM) under saturating levels of calcium, magnesium and zinc, monitored by tryptophan fluorescence. (A) Denaturation profiles of Ca2+-free NCS-1 in the presence of 1 mM EDTA, 1 mM Mg2+, 100 μM Zn2+, or 1 mM Mg2+ and 100 μM Zn2+. (B) Denaturation profiles of Ca2+-bound NCS-1 (100 μM Ca2+) in the absence, and in the presence of 100 μM Zn2+. Standard deviation of λmax values did not exceed 0.3 nm.
