Table 2.
Thermodynamic parameters of binding of D2R peptide to NCS-1 in 20 mM Tris-HCl buffer (pH 8.0), 150 mM NaCl, 5 mM CaCl2 in the presence or in the absence of 100 μM ZnCl2.
Ca2+ | Ca2++Zn2+ | |
---|---|---|
N | 0.676 ± 0.091 | 0.508 ± 0.102 |
KA, M-1 | (3.320 ± 0.568) × 104 | (11.800 ± 2.550) × 104 |
KD, M | 30.12 × 10-6 | 8.47 × 10-6 |
ΔH, kcal M-1 | -3.0 ± 0.5 | -3.2 ± 0.8 |
ΔS, cal K-1 M-1 | 10.6 | 12.4 |
ΔG, kcal M-1 | -6.2 | -6.9 |