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. 2018 Nov 29;21:101063. doi: 10.1016/j.redox.2018.11.021

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© 2018 Published by Elsevier B.V.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 1 — Generation ofNoxa1^-/-mice. A, Schematic representation of targeting vector containing LoxP/FRT-Neo cassette used for Noxa1 gene deletion. Location of Southern blot probe (PB5/6) and genotyping primers (WT1, NDEL1, and NDEL2) is shown in relation to wild-type (WT), knock-out Neo present (KO Neo⁺), and knock-out Neo-deleted (KO Neo-del) of Noxa1 gene exon structure (E1-E12). B, Southern blot analysis of genomic DNA fragments generated after BamHI digestion from Noxa1 wild-type (+/+), heterozygous (+/-), and knock-out (-/-) mice using PB5/6 probe. C, PCR analysis of genomic DNA extracted using genotyping primers WT1, NDEL1, and NDEL2. D, Representative images transverse aortic sections from wild-type and Noxa1^-/- mice stained with H&E (top panel) or immunohistochemistry using NOXA1 antibody (bottom panel). Scale is 100 µm.