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. 2018 Dec 14;9:3085. doi: 10.3389/fmicb.2018.03085

FIGURE 3.

FIGURE 3

Aspartic acid residues 46 and 51 of SaeR are important for the hemolysis of human erythrocytes by USA300. (A) Zone of hemolysis caused by USA300, an isogenic deletion mutant of saeR/S in USA300 (USA300ΔsaeR/S), or USA300 expressing SaeR with aspartic acid to alanine substitutions at residue 46 (ΔD46A), 51 (ΔD51A), or 61 (ΔD61A) during growth on agar containing 2.5% human red blood cells. (B) Percent hemolysis of human red blood cells in solution exposed to 6 h supernatants from USA300, USA300ΔsaeR/S, ΔD46A, ΔD51A, ΔD61A or ΔD46A and ΔD51A complemented with a plasmid expressing wt SaeR (ΔD46A+Comp or ΔD51A+comp, respectively). All panels represent at least 3 separate experiments. Panels (B,C) show the mean ± SEM with ∗∗P ≤ 0.01 and ∗∗∗∗P ≤ 0.0001 relative to USA300 as determined by one-way ANOVA with Dunnett’s multiple comparison test.