Fig. 3.

Intra-articular (tibiofemoral) delivery of VEGF receptor inhibitors did not affect the development of pain-associated behaviors in the CFA model of inflammatory monoarthritis. Intra-articular (i.a) CFA (single dose) plus two i.a. vehicle injections (arrow heads) caused a significant reduction in ipsilateral mechanical stimulus withdrawal threshold (a,d) at all time points measured, weight borne on ipsilateral hind paw (b,e) (days 1–10) and a significant increase in tibiofemoral joint diameter (c,f) compared to respective baseline (day 0). CFA + two i.a. injections of VEGF-A₁₆₅b or PTK787 (arrow heads) had no effect on the development of the pain-associated behaviors and joint diameter (a-f). Two statistical analyses were performed: 2-way ANOVA + Dunnett’s multiple comparisons tests: CFA + vehicle vs. respective baseline, *p < 0.05, **p < 0.01, ***p < 0.0001; or CFA + vehicle vs. CFA + drug, #p < 0.05, ##p < 0.01, ###p < 0.0001. A-C n = 10, D-F n = 5. Data displayed as mean ± SD.