Fig. 9.

Endothelial VEGFR2 knock-out affected pain behaviors in comparison to tamoxifen-treated Tie2CreER^T2-wildtype control animals. In addition to the knock-out and uninduced experimental groups (refer to Fig. 8, a tamoxifen-treated Tie2CreER^T2-negative (wildtype) group was included in the peri-articular CFA experiment. A significant reduction in ipsilateral (a) and contralateral (b) mechanical stimulus threshold developed in these mice as was observed in the uninduced control group (refer to Fig. 8. These effects were significantly different from the knockout group (ipsi: day 2, contra: all time points). A significant inhibition of the CFA-induced shift in weight bearing was also observed on day 2 (c) while no difference was observed in joint swelling (d). Statistical analyses: two-way repeated measures analysis of variances + Dunnett’s multiple comparisons test; either control vs. respective baseline (day 0): *p < 0.5, **p < 0.01, ***p < 0.001, ****p < 0.0001; or test groups vs. respective control at given time point: ##p < 0.01, ###p < 0.001, ####p < 0.0001, n = 5–6. Data displayed as mean ± SD.