Fig. 7.

IgSF9b deletion does not affect inhibitory synapses in the BA. a, b Schematic diagram illustrating recording sites in the BA, mean mIPSCs and representative mIPSC traces from the BA of WT, Nlgn2 KO, IgSF9b KO, and double KO mice. c Average cumulative distribution of mIPSC inter-event intervals and quantification of mean mIPSC frequency in the BA. d Probability distribution of mean mIPSC frequency of each analyzed cell in the BA. Kolmogorov–Smirnov test: WT vs. Double KO, p < 0.0001. e Average cumulative distribution of mIPSC amplitudes and quantification of mean mIPSC amplitude in the BA. f Probability distribution of mean mIPSC amplitude among all analyzed cells in the BA. n = 13–18 cells/5–6 mice per genotype. g–j Photomicrographs and quantification of the number of perisomatic puncta of g VIAAT, h gephyrin, i GABA_ARγ2, and j GABA_ARα1 in all four genotypes. Scale bar, 2 μm. n = 3–8 per genotype. Statistically significant ANOVA comparisons are marked in gray at the top of the panels and are listed in Table 1. For all other ANOVA comparisons F < 1. Post-hoc analysis: *p < 0.05 relative to WT, **p < 0.01 relative to WT, ***p < 0.001 relative to WT, #p < 0.05 relative to double KO. Error bars represent SEM. Box plots represent median and 25^th and 75^th percentiles and whiskers are drawn from the minimum to the maximum value. WT, white bars; Nlgn2 KO, red bars; IgSF9b KO, blue bars; double KO, purple bars