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. 2018 Dec 20;8:18006. doi: 10.1038/s41598-018-36324-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Maximum likelihood phylogeny of cowpea nodulating rhizobia from Ghana and South Africa based on concatenated sequences of atpD-glnII-gyrB-rpoB genes. The evolutionary history was inferred by using the Maximum Likelihood method based on the Kimura 2-parameter model⁵⁰. The percentage of trees in which the associated taxa clustered together is shown next to the branches. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. The analysis involved 40 nucleotide sequences. We used only those strains which were present in all test gene phylogenies. Codon positions included were 1st + 2nd + 3rd + Noncoding. All positions containing gaps and missing data were eliminated. There were a total of 1523 positions in the final dataset. Evolutionary analyses were conducted in MEGA7⁴⁹.