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. 2018 Dec 20;8:18006. doi: 10.1038/s41598-018-36324-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Maximum likelihood molecular phylogenetic analysis of cowpea nodulating rhizobia from Ghana and South Africa based on nifH gene sequences with type strains of Bradyrhizobium species. The evolutionary history was inferred by using the Maximum Likelihood method based on the Kimura 2-parameter model⁵⁰. The scale bar indicates the number of substitutions per site. The percentage of trees in which the associated taxa clustered together is shown next to the branches. The analysis involved 64 nucleotide sequences. Codon positions included were 1st + 2nd + 3rd + Noncoding. All positions containing gaps and missing data were eliminated. Evolutionary analyses were conducted in MEGA7⁴⁹.