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. 2018 Dec 14;9:3053. doi: 10.3389/fmicb.2018.03053

FIGURE 1.

FIGURE 1

Greater than two-thirds of global PolA diversity captured in a single Chesapeake Bay sample. (A) PolA peptide clusters (75% amino acid identity) among the SERC hybrid assembly and MgOl libraries. Each node within the diagram represents one PolA cluster and is colored by 762 position type (purple – Leu762; blue – Tyr762; red – Phe762; gray – mixture of 762 types in a cluster). (B) Unrooted maximum likelihood tree with ten bootstrap replicates of partial-length (E. coli N675-L799) PolA peptide representative sequences from the SERC hybrid assembly and 86 MgOl libraries clustered at 75%. Line colors represent cluster members source(s), and ring color denotes 762 position grouping based on 762 position residue within the PolA sequences.