Fig. 4.

L-ASC and AUR combination results in iron-mediated oxidative damage and cytotoxicity. A. Raji-HyPer3 cells were pre-loaded for 1 h with indicated concentrations of deferoxamine (DFO), washed extensively with PBS, and treated with 200 µM L-ASC and 0.5 µM AUR. To evaluate intracellular H₂O₂ levels, the intensity of HyPer3 was assessed at indicated time points by flow cytometry. Means ± SD from two experiments (n = 4) normalized to untreated control are shown for each time point. B. Raji cells were pre-loaded with DFO as described in A. and treated with 200 µM L-ASC and 0.5 µM AUR. The H2A.X phosphorylation, a marker of DNA damage, was assessed upon 6 h of the incubation. Bars are means from three experiments + SD. Statistical significance was evaluated using 1-way ANOVA test with Tukey's correction in DFO-containing groups and AUR+L-ASC without DFO; *p < 0.05 (left panel). Raji cells were pre-loaded with DFO as described in A. and treated with 200 µM L-ASC and 0.5 µM AUR. PI staining was used to assess % of dead cells after 48 h of incubation. Bars are means from two experiments + SD (right panel). Statistical significance was evaluated using 1-way ANOVA test with Tukey's correction in DFO-containing groups and AUR+L-ASC without DFO; ****p < 0.0001.