REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit ACTN4	Abcam	Cat# ab108198; RRID: AB_10858236
Rabbit ARP3	Abcam	ab151729
Rabbit CORTACTIN	Cell Signaling	Cat# 3503; RRID: AB_2115160
Rabbit ITGB1	Santa Cruz	Cat# M-106; RRID: AB_2130101
Guinea pig NEPHRIN	Progene	Cat# Gp-N2
Rat Nidogen (ELM1)	Nordic-MUbio	Cat# X1778M
Rabbit NMHC-IIA	Covance	Cat# PRB-440P; RRID: AB_291638
Rabbit NWASP	Cell Signaling	Cat# 4848S; RRID: AB_10694415
Mouse PAXILLIN	BD Transduction Lab.	Cat# 610051
Mouse PDH E2/E3	Abcam	Cat# ab110333; RRID: AB_10862029
Rabbit P-MLC	Cell Signaling	Cat# 3671/3674; RRID: AB_2147464
Rabbit PODOCIN	Santa Cruz	Cat# P0372; RRID: AB_2267439
Mouse SYNPO	Progene	Cat# 65194
Mouse Tubulin	Sigma	N/A
Mouse Vinculin	Abcam	Cat# SPM227; RRID: AB_883461
Rabbit WAVE2 (WASF2)	Atlas Antibodies	Cat# HPA045288; RRID: AB_2679287
Mouse WT-1	Millipore	Cat# 05-753-clone 6F-H2; RRID: AB_309966
Rabbit YAP	Cell Signaling	Cat# 14074S; RRID: AB_2218911
Rabbit ZYXIN	Atlas Antibodies	Cat# HPA004835; RRID: AB_1080771
Cy™3 AffiniPure Donkey AntiGuinea Pig IgG (H+L)	Jackson Immuno	Cat# 706-165-148
Alexa fluor (488,555,633 – resp. species)	Thermo Fisher Scientifc	N/A
DAPI	Thermo Fisher Scientifc	Cat# D21490
LTG	Vector Laboratories	Cat# FL-1321; RRID: AB_2336559
DBA	Vector Laboratories	Cat# RL-1032; RRID: AB_2336396
Alexa Fluor Phalloidin (546, 488)	Thermo Fisher Scientifc	Cat# A22283, A12379; RRID: AB_2315147
Biological Samples
All biological samples were generated in this study and described in detail in the Results	University Medical Center Freiburg	N/A
Chemicals, Peptides, and Recombinant Proteins
Blebbistatin	Sigma-Aldrich	B0560
Calyculin A	Sigma-Aldrich	C5552
CK312	Calbiochem	182518
CK548	Sigma-Aldrich	C7499
CK636	Sigma-Aldrich	C7374
CK666	Sigma-Aldrich	SML0006
CK689	Calbiochem	182517
CK869	Calbiochem	182516
Cytochalasin D	Sigma-Aldrich	C8273
Latrunculin A	Sigma-Aldrich	L5163
Nocodazole	Sigma-Aldrich	M1404
NSC23766	Sigma-Aldrich	SML0952
SMIFH2	Sigma-Aldrich	S4826
Y27632	Calbiochem	688000
Doxycycline hydrochloride	Fagron, Germany	803873
Prolong Gold Antifade	Thermo Fisher Scientifc	P36930
Ibidi Mounting Medium	Ibidi	50001
μ-Slide 8 Well, Collagen IV: #1.5 polymer	Ibidi	80822
μ-Dish 35 mm, high, ibiTreat	Ibidi	81156
μ-Dish 35 mm, high Glass Bottom	Ibidi	81158
Nunc Lab-Tek Chamber Slide system 8-well	Sigma-Aldrich	C7182
BioFlex Culture Plate Collagen IV	Flexcell	BF-3001C-IV
Fibronectin	Corning	354008
Collagen IV from human placenta	Sigma-Aldrich	C5533
Matrigel	Corning	356231
Bovine Type I Atelo-Collagen Solution	AdvancedBioMatrix	5005
40% Acrylamide	Bio-Rad	161-0140
2% Bis-acrylamide	Bio-Rad	161-0142
DSP	Sigma-Aldrich	803200
DPPB	Sigma-Aldrich	16646
Sulfo-SANPAH	ThermoFisher Scientific	22589
FluoSpheres	ThermoFisher Scientific	F8794
Collagenase, Type 2	Worthington Biochemical	LS004176
Protease from Streptomyces griseus	Sigma-Aldrich	P6911
DNase I	AppliChem	A3778
Critical Commercial Assays
MICROFLUORAL Mikroalbumin-Test	Progen	PR2005
Creatinine PAP LT-SYS	LT-Sys	LT-CR 0106
Deposited Data
Raw and analyzed mass spectrometry proteomics data	This paper	ProteomeXchange Consortium via PRIDE partner repository with the dataset identifier PXD010626 and PXD010601 For reviewer access: Username: reviewer06821@ebi.ac.uk Password: K0FQ12Zk And Username: reviewer05438@ebi.ac.uk Password: 2NoS9SGe
Experimental Models: Cell Lines
Immortalized human podocytes (AB8/13)	M. Saleem, Bristol University	N/A
Primary mouse podocytes (isolated from Arp3 and N-WASP conditional mice)	this study	N/A
Experimental Models: Organisms/Strains
mouse: N-WASPflox/flox	Cotta-de-Almeida et al. (2007)	N/A
mouse: Arp3flox/flox	Arnold HH, TU Braunschweig	N/A
mouse: Six2-Cre	Kobayashi et al. (2008)	N/A
mouse: Nphs2∗Cre	Moeller et al. (2003)	N/A
mouse: hNphs2∗rtTA∗TetOCre	Shigehara et al. (2003)	N/A
mouse: Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J	Muzumdar et al. (2007)	N/A
mouse: PAX8∗rtTA∗TetOCre	Traykova-Brauch et al. (2008)	N/A
Oligonucleotides
Genotyping PCR primers (Arp3) Fw: AGGGACAGTCTGTGTTTCTCCTAATATC Rev: AGGTGTTGACAACATGCTAAGAGCT	Eurofins	N/A
Genotyping PCR primers (NWASP) Fw: CACTTCTTTGCCCACATACA Rev: TAAAATGGCAGTGACAATGATGAC	Eurofins	N/A
Genotyping PCR primers (Nphs2Cre, Six2Cre) Fw: GCATAACCAGTGAAACAGCATTGCTG Rev: GGACATGTTCAGGGATCGCCAGGCG	Eurofins	N/A
Genotyping PCR primers (Pax8rtTA) Fw: CCATGTCTAGACTGGACAAGA Rev: CTCCAGGCCACATATGATTAG	Eurofins	N/A
Genotyping PCR primers (Nphs2rtTA) Fw: CGCACTTCAGTTACTTCAGGTCCTC Rev: GCTTATGCCTGATGTTGATGATGC	Eurofins	N/A
Genotyping PCR primers (Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J) Fw: CTCTGCTGCCTCCTGGCTTCT Rev(wt): CGAGGCGGATCACAAGCAATA Rev(mut): TCAATGGGCGGGGGTCGTT	Eurofins	N/A
Software and Algorithms
Graph Pad Prism 6 Software	GraphPad Software	https://www.graphpad.com/scientific-software/prism/
Fiji Image Analyzer	FIJI	https://fiji.sc/
Cytoscape 3.2.1	Cytoscape	http://www.cytoscape.org/