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. 2018 Oct 15;293(50):19213–19227. doi: 10.1074/jbc.RA118.005432

Figure 4.

Figure 4.

Fluorescence titration of pyrene-labeled hE2o(1–173) di-domain and pyrene-labeled hE2o(144–386) core domain by hE1o. A, quenching of the fluorescence of pyrene-labeled hE2o(1–173) di-domain by hE1o. Pyrene-labeled hE2o(1–173) di-domain (1.5 μm active centers) in 30 mm KH2PO4 (pH 7.5) was titrated by hE1o (0.013–0.261 μm). B, enhancement of the fluorescence of the pyrene-labeled hE2o(144–386) core domain on hE1o binding. Pyrene-labeled hE2o(144–386) core domain (1.5 μm) in 30 mm KH2PO4 (pH 7.5) was titrated by hE1o (0.013–0.261 μm).