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Spectral changes induced in the I105F mutant of T. cruzi CYP51 by obtusifoliol. Red, low-spin water-bound ferric P450; Soret band maximum, 417 nm. Blue, >85% high-spin substrate bound ferric P450; Soret band maximum, 393 nm. The increase in the absorbance at 280 nm is due to HPCD, which was used to dissolve the sterol.
