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. 2018 Oct 18;293(50):19317–19329. doi: 10.1074/jbc.RA118.005498

Figure 1.

Figure 1.

ZEB1 was up-regulated in hESC-derived neural cells. A, a scheme of neural differentiation of hESCs. Scale bar: 50 μm. B, real-time PCR analysis showed the alteration of ZEB1 mRNA levels during neural differentiation of hESCs. The amount of mRNA was normalized to GAPDH levels. Bars represent mean ± S.D. of three experimental replicates. C, Western blotting showed expression of the ZEB1 protein at different stages of neural differentiation from hESCs. β-ACTIN was a loading control; n = 3. D, representative images of immunostaining results using indicated antibodies in hESC-derived neural cells. DAPI was included to show the nuclei (here and after); n = 3. Scale bar: 50 μm. hESC, human embryonic stem cell; EB, embryoid bodies; NSC, neural stem cell; E8, essential 8 medium; NIM, neural induction medium; NDM, neural differentiation medium.