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. 2018 Oct 18;293(50):19317–19329. doi: 10.1074/jbc.RA118.005498

Figure 2.

Figure 2.

ZEB1 was necessary for neuronal differentiation. A, Western blotting showed ZEB1 protein levels at days 0, 15, and 35 of neural differentiation from hESCs with or without ZEB1 knockout. β-TUBULIN was the loading control. B, phase-contrast images of the indicated cells during different days of neural differentiation; n = 3. Scale bar: 50 μm. C, left, flow cytometry analysis with BrdU antibody using cells after 4 days attachment of spheres for neuronal differentiation (day 30). Right, statistical analysis of the left results; n = 3. D, left, cell apoptosis and death analysis with PE Annexin V/7-AAD Kit. Right, statistical analysis to show the percentage of viable cells (Negative) or cells in early apoptosis (Annexin V+, p < 0.001), in late apoptosis or already dead (7-AAD+ and Annexin V+ and 7-AAD+, p < 0.001); n = 3. ZEB1-KO, ZEB1 knockout. OE (ZEB1-KO), overexpress ZEB1 in ZEB1-knockout cells.