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The effect of CNP overexpression on XBP1 splicing. A, HEK293T cells were transfected with control cDNA (FLAG-Sox2) or FLAG-CNP–expressing PB transposon. Upon ER stress, spliced and total XBP1 mRNA levels were analyzed by RT-qPCR. Error bars represent S.E. *, p < 0.05. Overexpression of CNP was confirmed by Western blot analysis. B, Western blot analysis of CNP, RtcB, and RtcA proteins in parental and CNP-overexpressing HEK293T cells and brain lysate. CTL, control.
