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. 2018 Oct 15;293(50):19177–19190. doi: 10.1074/jbc.RA118.003198

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© 2018 Cai et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 5. — miR-664-5p inhibits myogenic differentiation. A, overexpression efficiency of miR-664-5p mimics at 0, 2, and 4 days. B, relative mRNA expression of MyoG and MyHC after induction for 4 days. C, protein expression of MyoG and MyHC after induction for 4 days. D, quantitative results of protein expression in C. E, immunofluorescence staining of MyHC after induction for 4 days. F, statistical results of the myoblast fusion index after induction for 4 days. G, differentiation index of myoblast after induction for 4 days. Values are expressed as the means ± S.E. (n = 3). *, p < 0.05; **, p < 0.01, versus NC.