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. 2018 Oct 15;293(50):19177–19190. doi: 10.1074/jbc.RA118.003198

Figure 6.

Figure 6.

miR-664-5p targets Wnt1 during myogenic differentiation. A, Wnt1 is a candidate target gene of miR-664-5p. B, construction of the dual-luciferase reporter psiCHECK-2.0–Wnt1 3′ UTR. C, dual-luciferase reporter assay was performed by cotransfecting miR-664-5p mimics and WT or mutant vectors. The relative luciferase activity was presented as Renilla luciferase/firefly luciferase. D, relative expression profile of miR-664-5p during myogenic differentiation. *, p < 0.05; **, p < 0.01 versus 0 days. E, relative mRNA expression of Wnt1 after treatment with miR-664-5p mimics. F, Western blot analysis of Wnt1 protein expression after transfection with miR-664-5p mimics and the statistical results of Wnt1 protein expression at 4 days after myogenic induction. Values are expressed as the mean ± S.E. (n = 3). *, p < 0.05; **, p < 0.01, versus NC.