Figure 5.

The effect of the NFR on H1-dependent restriction of linker DNA accessibility does not extend to the entire array. A, schematic showing the 25-mer array, EcoRV sites (vertical arrows) and the T_Nuc (center; shaded). B, 25-mer arrays containing either a WT nucleosome or an NFR at the central (T_Nuc) position were incubated with or without H1 in buffer containing 10 mm Mg²⁺ as indicated and digested with EcoRV (2 units) for the indicated times, and then products were separated on SDS-agarose gels.