Figure 2.

MALDI-TOF/MS spectrum of the N-glycans released from IgGs of naïve ferrets. N-glycans released from the purified IgGs of pooled sera from four naïve ferrets were reduced, permethylated, and measured by MALDI-TOF-MS. The composition of the glycans was determined through a database search with GlycoMod. The corresponding detailed structures were investigated through HCD and CID fragmentation experiments. Among these structures, five pairs of isomers were identified by their differing retention times on C18 reverse-phase nanoLC, and their relative abundance was determined by the intensity of monoisotopic m/z peaks in LC-MS.