Skip to main content
. 2018 Dec 20;12(3):453–461. doi: 10.1016/j.tranon.2018.12.002

Figure 2.

Figure 2

Flow cytometry analysis. (A) Expression analysis of integrin αvβ3 and αvβ5, and NRP-1 in PC-3 cells by flow cytometry. Red line represented the isotype, and the blue line represented the sample. The flow cytometry results were analyzed using flowjo software 7.6.1. PC-3 (B) and RWPE-1 (D) cells (2 × 105/well) were cultured in 6-well plates and treated with 60 μg/ml of IL-24-iRGD or IL-24 for 48 hours. And then Annexin V-FITC/PI staining was used for cell apoptosis assay, and FITC-positive parts represent cell apoptosis, including lower right corners and top right corners. Quantitative representation of the proportion of cell apoptosis analyzed in PC-3 (C) and RWPE-1 (E). Data are expressed as the mean ± SD. ⁎P < .05, ⁎⁎P < .01, and ⁎⁎⁎P < .001, as indicated. ns, not significant.